荧光素(FAM)是比较古老的荧光染料之一。FAM的这种衍生物含有环辛炔基团(二苯并环辛炔，缩写为DBCO或ADIBO)，可以通过无铜、菌种促进的叠氮炔环加成(spAAc)与叠氮结合。
Fluorescein (FAM) is one of the oldest yet well known fluorescent dyes. This derivative of FAM contains cyclooctyne group (dibenzocyclooctyne, abbreviated as DBCO or ADIBO) for the conjugation with azides via copper-free, strain-promoted azide alkyne cycloaddition (spAAc).
中文名称：5-羧基荧光素二苯并环辛炔
英文名称：5-FAM DBCO；FAM DBCO,6-isomer
CAS：1384485-04-4
外观：橙色的固体
分子式：C39H26N2O7
分子量：634.64
纯度：95%+
溶解性：溶于DMSO, DMF，水
储存条件：-20℃避光保存
用途：仅用于科研，不能用于人体
结构式：
FAM DBCO可用于标记蛋白质、多肽、核酸和其他含有叠氮基团的分子。叠氮化物与DBCO简单混合即可自发反应。该反应不需要任何催化剂，反应效率很高。
FAM DBCO can be used for the labeling of proteins, peptides, nucleic acids, and other molecules containing azide groups. Azides react with DBCO spontaneously upon simple mixing. The reaction does not require any catalysts, and it is very efficient.
荧光素可以使用各种不同的仪器进行检测。
Fluorescein can be detected using a variety of different instruments.
